Question

PCR utilizes specific DNA primers and a thermo stable polymerase enzyme to amplify (make many copies of) a particular DNA sequence (typically 100-600 bases long). Double stranded DNA (dsDNA) must first be separated and primers anneal (complimentary base pair to) their targeted sequences. Polymerase can then extend the DNA strands. What must also be added to the mixture besides primers and polymerase enzymes in order to have amplification of DNA?

A.ATP.
B.RNA polymerase.
C.dNTPs.
D.electron carriers.
E.DNA repair enzymes.

Answer 1

C.dNTPs.

Step-by-step explanation:

The dNTPs (deoxynucleoside triphosphate) are made of a 5-carbon sugar (a deoxyribose), three phosphate groups and a nitrogenous base (adenine, thymine, guanine or cytosine).

They are the monomers of DNA used in PCR reactions to synthesize the new strands. There are four dNTPs, each containing a different nitrogenous base (dATP, dTTP, dGTP and dCTP). The DNA polymerase will extend the primers by adding a dNTP complementary to the template strand during the elongation phase of PCR.