Question

Drag each ingredients of a PCR reaction into the bin labeled with the step in the PCR cycle in which it is first used Reset Help Denaturation (95 degrees C) Annealing (55 degrees C) Elongation (65 degrees C) Taq polymerase primers template DNA dNTPs (deoxyribonuleotieds)

Answer 1

PCR steps

Step-by-step explanation:

Put all the ingredients toghether in an eppendorf in the thermocycler.

Steps of PCR will be:

1-Denaturation (95°C): DNA template will denaturalize and the two strands will get apart (with no more hydrogen bonds between them)

2-Annealing (55°C): primers will pair with each DNA single-strand (annealing temperature must be high enough to allow the correct annealing)

3-Elongation (65°C): Taq polymerase will make a complementary strand with the dNTPs (complementary to each single-DNA strand)

(reset and help)