Question

We can estimate the relative mobility of a population of molecules along the surface of a living cell by fluorescently labeling the molecules of interest, bleaching the label in one small area, and then measuring the speed of signal recovery as molecules migrate back into the bleached area. What is this method called?

(a) SDS
(b) SPT
(c) GFP
(d) FRAP

Answer 1

Answer: FRAP

Step-by-step explanation:

FRAP, fluorescence recovery after photobleaching is a technique which is used to find the kinetics of diffusion through the cells.

This technique is very useful for studying the cell's protein binding and membrane diffusion.

This technique is helpful in quantifying the lateral diffusion of a thin film containing fluroscently labelled probes.