The correct answer is: Include another aquarium that has normal salinity to compare to the low salinity aquarium
An aquarium with normal salinity would be a negative control.
It is desirable to have a negative control in an experiment because it uses the same components as a primary experiment (performed at the same time on a different population) with no treatment. Negative control is predicted to produce no change to results of interest to the experiment it is only to be compared to.